Practical 2 Drawings

Practical 2 Drawings
400X Magnification of Gastic Mucosa
100X Magnification of Gastric Mucosa
Practical 3
01.What is the expected size of the protein being detected by the sera from pernicious anaemia
patients?
-Serum samples of patients with pernicious anaemia, has antibody to react with 95 KDa a-subunit and 60-
90kDa glycosylated B-subunit. So, the expected size of the protein being detected should be in the range of
60-90kDa or above 90kDa.when using the precisions plus protein kaleidoscope standards #161 -0375
marker the bands above 75kDa are a and below 75 (should be closer to 75) are B(Dow et al 1985).
02.Which subunit of the proton pump does this correspond to?
Alpha subunit of the ATPase proton pump 95kDa
03. Describe another method that could be used to detect the quantitate the levels of anti-proton
pump antibodies in patient sera?
-Flow microparticle immunofluorescence assay (Buhling et al 2004)
-Microparticle based flow cytometric assay used to determine the level of soluble markers. This assay is to
measure large number of analytes at the same reducing assay time. Assay is based on antigen-antibody
interactions.
04. The secondary antibody that was used in the western and immune-histochemistry as a sheep
anti-human Ig-HRP conjugate. What is the antigen that this antibody is specific for? What animal
was this antibody raised in? what is the enzyme that is conjugated to the antibody?
– Antigen affinity – purified
– Sheep
– Peroxidase

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